Pictured above from left to right: BRG members 2016, Catherine Florentine, Demetria DeBartolo, Emma Henricks, Bruce Branchini, Franceine Welcome, and Tara Southworth. Not pictured: Danielle Fontaine.
-Structure and Mechanistic Studies on Firefly Luciferase (with Andrew Gulick, Hauptman-Woodward Institute)
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Pictured above, two conformations of P. pyralis luciferase provided by Andrew Gulick. At left, Luciferase-DLSA in Adenylation Conformation and at right, BMOE Cross-Linked Luciferase-DLSA in Oxidation Conf 2
- Development of Chimeric Luciferases with Enhanced Activity and Stability
- Directed Evolution Studies to Produce Optimized Luciferase-Substrate Analog Systems
- Development of nearIR emitting firefly luciferin analogs and luciferases (with Jim Anderson, University College London, and Amit Jathoul, Cardiff University)
- Biochemical Characterization of the Orfelia fultoni bioluminescence system (with Vadim Viviani, Dept. Physics, Chemistry and Mathematics (DFQM) 91³ΙΘΛTS, and Ilia Yampolsky, Institute of Bioorganic Chemistry Russian Academy of Sciences and coworkers)
- Development of novel luciferin and luciferases for bioluminescence imaging applications (with Roberto Speck and Audrey Fahrny, Department of Infectious Diseases University Hospital of Zurich)
- Bioanalytical Applications of Luciferase Variants (with Aldo Roda, University of Bologna)
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The above images demonstrate (left) an Orfelia fultoni larva photographed in the dark emitting blue light and (right) bioluminescence imaging of mouse tumors.
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